By Olaus Johan Murie
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Additional resources for A Field Guide to Animal Tracks
Pyromaker provides a virtual tool to show all possible Pyrogram patterns for different mutations at a certain dispensation order for educational purposes. Different dispensation sequences can produce totally different Pyrograms or identical DNA sequences, so often it is critical to design an optimal dispensation sequence in order to get clear and distinctive identification of all possible sequence changes before Pyrosequencing is actually performed. To assist with all the aspects mentioned above, computer programs working directly or indirectly on actual Pyrograms are developed.
1] and references therein). One reason for these inconsistencies is the often forgotten truth that the efforts one has to invest for defining the performance characteristics of a quantitative analytical assay depend substantially on what one wants to show and what one has to show. For example, the physiological and pathological levels of glucose or cholesterol in the blood in humans are so far above the detection threshold of the nowadays routinely applied analytical tools that it is not necessary to exactly determine the limit of detection for these clinically very important parameters.
3b) ahead of the wild-type T, one must replace the G in the 12b position (TGT) with a second T, so ending up with TTT (Fig. 5a, middle left). Alternatively, the T in the 12c position (TGT) can be replaced with a G to produce 4 Gs in a row (TGG/GGC, codons 12/13, Fig. 5a, middle right), thereby moving the T peak into the G position. Both sequences produce the three signature peaks shown in the clinical Pyrogram (Fig. 3b), but only the TTT Pyrogram yields the correct peak height ratios for the three novel peaks (3:1:1, respectively).